Person

FH-Prof. DI Dr. Jaroslaw Jacak

Nanolitography, Fluorescent Microscopy and Spectroscopy, Biotechnology, STED Microscopy and Lithography, Biomaterials, Laser Optics , Single molecule scanning and analysis techniques

Research interests

  • Laser Optics
  • Nanolitography
  • Biomaterials
  • Single molecule scanning and analysis techniques
  • STED Microscopy and Lithography
  • Biotechnology
  • Fluorescent Microscopy and Spectroscopy
2018
B. Buchegger, J. Kreutzer, M. Axmann, S. Mayr, R. Wollhofen, B. Plochberger, J. Jacak, T. Klar - Proteins on Supported Lipid Bilayers Diffusing around Proteins Fixed on Acrylate Anchors - ANALYTICAL CHEMISTRY,… more
2018
F. Hauser, S. Mayr, A. Tauscher, J. Jacak - Statistical analysis of 3D localization microscopy data for quantification of protein distribution upon clot formation - Proceedings of 8th Single Molecule Localization… more
2018
S. Mayr, F. Hauser, A. Peterbauer, A. Tauscher, C. Naderer, M. Axmann, B. Plochberger, J. Jacak - Localization Microscopy of Actin Cytoskeleton in Human Platelets - International Journal of Molecular Sciences,… more
2018
F. Hauser, S. Mayr, S. Puthukodan, A. Tauscher, T. Klar, J. Jacak - Nanometer Tomography of Cells - Proceedings of the First INTERREG - 6th TERMIS Winterschool 2018, Radstadt, Salzburg, Austria, 2018 more
2017
S. Mayr, M. Brodesser, F. Hauser, J. Breuss, M. Aspetsberger, D. M. Borgmann, S. M. Winkler, C. Gabriel, E. Priglinger, J. Jacak, B. Plochberger - A FAST AND RELIABLE ONLINE-SYSTEM FOR PLATELET VIABILITY… more
2017
R. Wollhofen, J. Jacak, T. Klar - Two photon laser induced molecular patterning - Proceedings of the XIX Annual Linz Winter Workshop 2017, Linz, Austria, 2017 more
2017
R. Wollhofen, B. Buchegger, J. Jacak, J. Kreutzer, T. Klar - Functional photoresists for sub-diffraction stimulated emission depletion lithography - OPTICAL MATERIALS EXPRESS , Vol. 7, No. 7, 2017, pp.… more
2017
C. Plamadeala, R. Wollhofen, J. Jacak, J. Heitz, T. Klar - Bioinspired polymer microstructures for directional transport of oily liquids - Royal Society Open Science, Vol. 10, No. 1098, 2017 more
2017
G. Langer, B. Buchegger, J. Jacak, T. Klar, T. Berer - Frequency domain optical resolution photoacoustic and fluorescence microscopy using a modulated laser diode - SPIE BiOS 2017 , San Francisco, United… more
2017
J. Kreutzer, B. Buchegger, J. Jacak, T. Klar, R. Wollhofen - 3D STED lithography: Protein binding and non-binding - SPIE BiOS 2017 , San Francisco, United States of America, 2017 more
2017
B. Plochberger, M. Brodesser, F. Hauser, J. Breuss, C. Gabriel, J. Jacak, S. M. Winkler, D. M. Borgmann, A. Hangler, M. Aspetsberger, L. Bindreiter - A Fast and Reliable Online-System for Platelet Viability… more
2017
B. Plochberger, M. Brodesser, F. Hauser, M. Axmann, J. Jacak, S. M. Winkler, L. Bindreiter, M. Aspetsberger - A Fast and Reliable Online-System for Platelet Viability Studies - ÖGMBT Meeting 2017, Innsbruck… more
2017
S. Mayr, A. Tauscher, F. Hauser, C. Lindner, B. Plochberger, C. Gabriel, H. Stangl, H. Redl, J. Jacak - Three-dimensional nanoscopic visualization of platelet membrane proteins in a white clot in-vitro… more
2017
G. Langer, B. Buchegger, J. Jacak, T. Klar, T. Berer - Multimodal fluorescence and photoacoustic microscopy in the frequency domain - SPIE BiOS 2017 , San Francisco, United States of America, 2017 more
2017
F. Hauser, S. Mayr, B. Buchegger, T. Klar, B. Plochberger, J. Jacak - 3D dSTORM Algorithms Improvement by Application of Geodesic Acceleration - Proceedings of the XIX Annual Linz Winter Workshop 2017,… more
2017
B. Buchegger, J. Kreutzer, T. Klar, J. Jacak - Nanostructured functional polymers for selective protein binding - 2017 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference… more
2017
J. Jacak, T. Klar, J. Kreutzer, R. Wollhofen - Sub-Diffraction STED Lithography using Orthogonally Functionalized Resins - BIOPHYSICAL JOURNAL, Vol. 112, No. 3, 2017 more
2017
M. Axmann, R. Wollhofen, P. Freudenthaler, C. Gabriel, T. Klar, J. Jacak - Multiphoton Polymerized 3D Protein Assay -  ACS Applied Materials & Interfaces, Vol. 10, No. 2, 2017, pp. 10-18 more
2016
B. Buchegger, J. Kreutzer, B. Plochberger, R. Wollhofen, J. Jacak, G. Schütz, U. Schubert, T. Klar - Stimulated Emission Depletion Lithography with Mercapto-Functional Polymers - ACS Nano, Vol. 1021,… more
2016
B. Aekbote, T. Fekete, J. Jacak, G. Vizsnyiczai, P. Ormos, L. Kelemen - Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells - Biomedical Optics Express, Vol.… more
2016
J. Jacak, R. Wollhofen, T. Klar - Functional polymer scaffolds for biomolecule immobilization - SPIE Photonics Europe Proc., Brüssel, Belgium, 2016, pp. 1-6 more
2016
J. Jacak, R. Wollhofen, T. Klar - STED lithography for applications in biology - Proc. Photonics West Conference 9759 (Advanced Fabrication Technologies for Micro/Nano Optics and Photonics), San Francisco,… more
2016
G. Langer, B. Buchegger, J. Jacak, T. Berer - Frequency domain photoacoustic and fluorescence microscopy - Biomedical Optics Express, Vol. 7, No. 2692, 2016, pp. 37-43 more
2016
J. Jacak, R. Wollhofen, T. Klar - Nanoscopic structuring with STED lithography - SPIE Photonics Europe Proc., Brüssel, Belgium, 2016 more
2016
J. Heitz, M. Wiesbauer, R. Wollhofen, J. Jacak, T. Klar - Three-dimensional photonic structures on transparent substrates fabricated by two-photon polymerization for use as cell substrates and for wetting… more
2016
S. Mayr, D. M. Borgmann, H. Polin, S. Schaller, T. Endmayr, L. Obritzberger, V. Dorfer, C. Gabriel, S. M. Winkler, J. Jacak - Classification of Rhesus D Antigen Expression at Single Molecule Level by High-Resolution… more
2016
J. Heitz, C. Plamadeala, M. Wiesbauer, R. Wollhofen, J. Jacak, T. Klar - Bone-forming cells with pronounced spread into the third dimension in polymer scaffolds fabricated by two-photon polymerization… more
2016
B. Plochberger, M. Brodesser, F. Hauser, S. Mayr, J. Jacak, D. M. Borgmann, S. M. Winkler, L. Bindreiter, A. Hangler, M. Aspetsberger - Blood cell viability study via real-time image analysis exemplified… more
2016
J. Jacak, R. Wollhofen, T. Klar - Nano-Confined Polymer Structures for Protein Binding - BIOPHYSICAL JOURNAL, Vol. 110, No. 3, 2016 more
2016
B. Plochberger, R. Wollhofen, J. Jacak, M. Axmann, G. Schütz, T. Klar - Confinement of Lipid Membranes by Nanostructured Polymer Patterns for Cell to Cell Mimicking - BIOPHYSICAL JOURNAL, Vol. 110, No.… more
2015
T. Klar, B. Buchegger, R. Wollhofen, J. Jacak - STED-Lithography for Bio-Applications - Proc. CLEO/Europe - EQEC 2015, München, Germany, 2015, pp. 1-6 more
2015
J. Jacak, S. Schaller, D. M. Borgmann, S. M. Winkler - Characterization of the Distance Relationship Between Localized Serotonin Receptors and Glia Cells on Fluorescence Microscopy Images of Brain Tissue… more
2015
S. M. Winkler, S. Schaller, D. M. Borgmann, V. Dorfer, M. Affenzeller, J. Jacak, H. Redl, J. Weghuber - Identification and classification of objects and motions in microscopy images of biological samples… more
2015
R. Wollhofen, J. Jacak, T. Klar - Laser induced protein patterning - DK nanocell Summerschool Proceedings, Strobl, Austria, 2015 more
2015
S. Puthukodan, J. Jacak, R. Wollhofen, T. Klar - Stimulated Emission Depletion Microscopy - DK nanocell Summerschool Proceedings, Strobl, Austria, 2015 more
2015
R. Wollhofen, B. Buchegger, J. Jacak, M. Wiesbauer, T. Klar - Sub 100 nm STED Lithography - DINAMO (Discussions on Nano & Mesoscopic Optics) 2015, El Chalten, Argentina, 2015 more
2015
D. M. Borgmann, S. Mayr, H. Polin, L. Obritzberger, S. Schaller, V. Dorfer, J. Jacak, S. M. Winkler - Classifying Human Blood Samples Using Characteristics of Single Molecules and Cell Structures on Microscopy… more
2015
B. Buchegger, J. Kreutzer, J. Jacak, B. Plochberger, R. Wollhofen, U. Schubert, G. Schütz, T. Klar - STED-Lithography with Mercapto-Functional Polymers - ACS Nano, Vol. 10, No. 9, 2015 more
2014
J. Jacak, M. Sams, L. Muresan, R. Silye, K. Schilcher - Spatial cluster analysis of nanoscopically mapped serotonin receptors for classification of fixed brain tissue - Journal of Biomedical Optics, Vol.… more
2014
R. Wollhofen, B. Buchegger, J. Jacak, T. Klar - Nano-Confined Polymer Structures for Adhesive Protein Binding - European Optical Society EOSAM 2014 Proc., Berlin, Germany, 2014 more
2014
J. Jacak, T. Klar, M. Wiesbauer, R. Wollhofen - Nano-confined polymer structures for protein adhesion - Proc. 2014 Photonics West Conference, San Francisco, United States of America, 2014 more
2014
J. Jacak - STED Lithography Structuring with Functional Clusters - Proceedings of XXVth IUPAC Symposium on Photochemistry, Bordeaux, France, 2014, pp. 2-7 more
2014
J. Jacak, J. Göhring, N. Fulcher, A. Barta - Suitable transfection methods for single particle tracing in plant suspension cells - Plant Methods, Vol. 10, No. 1, 2014 more
2014
J. Göhring, J. Jacak, A. Barta - Imaging of Endogenous Messenger RNA Splice Variants in Living Cells Reveals Nuclear Retention of Transcripts Inaccessible to Nonsense-Mediated Decay in Arabidopsis -… more
2013
J. Jacak, M. Wiesbauer, R. Wollhofen, T. Klar - Nano-confined Protein Anchors - Proceedings European Conferences on Biomedical Optics (ECBO) 2013, München, Germany, 2013 more
2013
J. Jacak, R. Wollhofen, T. Klar, M. Wiesbauer - Nano-confined protein anchors, structured by STED lithography, probed by dSTORM - Proc. DPG-Frühjahrstagung der Sektion Kondensierte Materie (SKM), Regensburg,… more
2013
J. Jacak, R. Wollhofen, T. Klar - Nano resolution and 55nm line width achieved in visible light STED-lithography - The European Conference on Lasers and Electro-Optics, Munich, Germany, 2013 more
2013
R. Wollhofen, J. Jacak, M. Wiesbauer, T. Klar - Applications of sub 100 nm STED Lithography in Nano- and Biophotonics - Proceedings EOS Topical Meeting on Optics at the Nanoscale (ONS'13), Capri, Italy,… more
2013
J. Jacak, C. Hrelescu, J. Heitz - Applications of Laser-Induced Periodic Surface Structures (LIPSS): Formation of Gold Nano- Wires and Activation of Biological Cells, Cultivated on Polymer Substrates … more
2013
J. Jacak, R. Wollhofen - Single Molecule Spectroscopy - SPIE BiOS 2013, San Francisco, United States of America, 2013 more
2013
S. Schaller, J. Jacak, M. Sams, S. M. Winkler - Statistical Analysis of the Relationship between Spots and Structures in Microscopy Images - Lecture Notes in Computer Science LNCS 8111, Las Palmas de Gran… more
2013
S. Schaller, J. Jacak, P. Bettelheim, D. Gschwandtner, S. M. Winkler - Analyzing Motion Characteristics of Single Molecules for Providing Evidence of PNH in Microscopy Images - Proceedings of the 21st… more
2013
S. Schaller, J. Jacak, D. Gschwandtner, P. Bettelheim, S. M. Winkler - Identification of PNH Affected Cells by Classifying Motion Characteristics of Single Molecules - Proceedings of the International… more
2013
J. Göhring, J. Jacak, N. Fulcher - TeloTool: A New Tool for Telomere Length Measurement from Terminal Restriction Fragment (TRF) Analysis with Improved Probe Intensity - NUCLEIC ACIDS RESEARCH, Vol. 42,… more
2013
J. Jacak, M. Wiesbauer, R. Wollhofen, B. Vasic, K. Schilcher, T. Klar - Nano-Anchors with Single Protein Capacity Produced with STED Lithography - Nano Letters , Vol. 13, No. 11, 2013 more
2013
R. Wollhofen, J. Jacak, J. Katzmann, C. Hrelescu, T. Klar - 120 nm resolution and 55 nm structure size in STED-lithography - Optics Express, Vol. 21, No. 9, 2013 more
2013
J. Jacak, H. Schnidar, L. Muresan, F. Aberger, G. Schütz, J. Hesse - Expression analysis of multiple myeloma cancer stem cells using single molecule microarray readout - JOURNAL OF BIOTECHNOLOGY, Vol.… more
2012
S. Schaller, J. Jacak, D. M. Borgmann, J. Weghuber, S. M. Winkler - An Image Analysis Suite for Automated Spot Detection in Cellular and Nano Structures of Microscopy Images - Proceedings of the 20th Annual… more
2012
J. Jacak, Z. Szegletes, P. Ormos, G. Schütz - Aminosilane-based functionalization of two-photon polymerized SU-8 microstructures - EUROPEAN POLYMER JOURNAL, Vol. 48, No. 10, 2012 more
2012
D. Borgmann, J. Weghuber, S. Schaller, J. Jacak, S. M. Winkler - Identification of Patterns in Microscopy Images of Biological Samples using Evolution Strategies - Proceedings of the 24th European Modeling… more
2012
S. Schaller, S. M. Winkler, J. Weghuber, M. Sams, J. Jacak - µDetect: Design of a Framework for Optimized Structure Analysis in Fluorescence Microscopy Images - Proceedings of 6th FFH 2012, Graz, Austria,… more
2011
R. Schmidt, J. Jacak, C. Schirwitz, V. Stadler, G. Schütz - Single-molecule detection on a protein-array assay platform for detection of a tuberculosis antigen - Journal of Proteome Research, Vol. 10,… more
2010
J. Jacak, C. Hesch, J. Hesse, G. Schütz - Identification of immobile single molecules using polarization- modulated asynchronous time delay and integration-mode scanning - ANALYTICAL CHEMISTRY, Vol. 82,… more
2010
J. Jacak, L. Muresan, J. Hesse, E. Klement, G. Schütz - Microarray analysis at single molecule resolution - IEEE transactions on nanobioscience , Vol. 9, No. 1, 2010 more
2009
J. Jacak, J. Hesse, C. Hesch, G. Schütz - Two-Stage Focus Hold System for Rapid Ultrasensitive Readout of Large Area Biochips - JOURNAL OF MICROSCOPY-OXFORD, Vol. 234, No. 3, 2009 more
2009
J. Jacak, J. Hesse, L. Muresan, F. Aberger, G. Schütz - Oligonucleotide Microarray Analysis with Single Molecule Sensitivity - BIOPHYSICAL JOURNAL, Vol. 96, No. 3, 2009 more
2007
B. Plochberger, M. Brodesser, F. Hauser, J. Jacak, S. M. Winkler, D. M. Borgmann, S. Mayr, J. Breuss, C. Gabriel, A. Hangler, M. Aspetsberger, L. Bindreiter - Blood cell viability study via real-time image… more
2006
J. Hesse, J. Jacak, S. Howorka, L. Muresan, F. Aberger, G. Schütz - RNA expression profiling at the single molecule level - GENOME RESEARCH, Vol. 16, No. 8, 2006 more
2005
J. Jacak, J. Hesse, C. Hesch, F. Aberger, S. Howorka, G. Schütz - Ultra–sensitive DNA detection on microarrays” - Proceedings of SPIE, Vol. 5699, No. 1, 2005 more
2004
J. Hesse, J. Jacak, A. Sonnleitner, O. Höglinger, G. Schütz - Single molecule reader for high-throughput bioanalysis - ANALYTICAL CHEMISTRY, Vol. 76, No. 19, 2004 more
2016
Within the interregional project Czech-Austrian Center for Supracellular Medical Research funded by the European Union we established a new facility regarding the production of blood vessel mimicking structures.Currently,… more
2016
2013
Fluorescence microscopy is going to become one of the core technologies for biomedical research and diagnostics. Therefore, image analysis is an increasing challenge for biomedical researchers. In the… more
2011
Receptor proteins on the surface of human cells play a key role in the transmission of signals from the environment into the interior of the cell. A complex network of these proteins controls all cellular… more