Localization Microscopy of Actin Cytoskeleton in Human Platelets
Publication, 2018
Outline
S. Mayr, F. Hauser, A. Peterbauer, A. Tauscher, C. Naderer, M. Axmann, B. Plochberger, J. Jacak - Localization Microscopy of Actin Cytoskeleton in Human Platelets - International Journal of Molecular Sciences, Vol. 19, No. 1150, 2018
Abstract
Here, we measure the actin cytoskeleton arrangement of different morphological states
of human platelets using a new protocol for photo-switching of rhodamine class fluorophores.
A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488
conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized
and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less
drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional
accuracy of 12 nm