Frequency domain optical resolution photoacoustic and fluorescence microscopy using a modulated laser diode
G. Langer, B. Buchegger, J. Jacak, T. Klar, T. Berer - Frequency domain optical resolution photoacoustic and fluorescence microscopy using a modulated laser diode - SPIE BiOS 2017 , San Francisco, United States of America, 2017
There is an increasing interest in functionalized complex 3D microstructures with sub-micrometer features for micro-and nanotechnology applications in biology. Depending primarily on the material of the structures various methods exist to create functional layers of simple chemical groups, biological macromolecules or metal nanoparticles. Here an effective coating method is demonstrated and evaluated on SU-8 based 3D microstructures made by two-photon polymerization. Protein streptavidin and gold nanoparticles (NP) were bound to the microstructures utilizing acid treatment-mediated csilane chemistry. The protein surface density, quantified with single molecule fluorescence microscopy revealed that the protein forms a third of a monolayer on the two-photon polymerized structures. The surface coverage of the gold NPs on the microstructures was simply con-trolled with a single parameter. The possible degrading effect of the acid treatment on the sub-micrometer features of the TPP microstructures was analyzed. Our results show that the silane chemistry-based method, used earlier for the functionalization of large-area surfaces can effectively be adapted to coat two-photon polymerized SU-8 microstructures with sub-micrometer features.
Frequency domain optical resolution photoacoustic and fluorescence microscopy using a modulated laser diode. Available from: https://www.researchgate.net/publication/314206400_Frequency_domain_optical_resolution_photoacoustic_and_fluorescence_microscopy_using_a_modulated_laser_diode