Atomic force microscopy-derived nanoscale chip for the detection of human pathogenic viruses.
Publikation, 2008
Outline
H. Artelsmair, F. Kienberger, A. Tinazli, R. Schlapak, R. Zhu, J. Preiner, J. Wruss, M. Kastner, N. Saucedo-Zeni, M. hoelzl, C. Rankl, W. Baumgartner, S. Howorka, D. Blaas, H. Gruber, R. Tampe, P. Hinterdorfer - Atomic force microscopy-derived nanoscale chip for the detection of human pathogenic viruses. - Small, 2008
Abstract
Native-protein nanolithography (NPNL) was used to fabricate stable
bioactive arrays of viral receptor spots. The arrays were specific for the
cognate virus and devoid of nonspecific protein and virus adsorption under
physiologic conditions. The spot size ranged from 200nm200nm to
2mm2mm and up to 33 spots were arranged per array. With proper
force adjustment in the patterning experiments, His6-tagged bovine serum
albumin (BSA) molecules were selectively removed from the underlying
self-assembled monolayer (SAM) while leaving the latter intact. Injection of
His6-tagged very low density lipoprotein receptor (VLDLR-His6)
constructs resulted in specific, oriented binding to the Ni2þ-loaded
bis-(nitrolotriacetic acid) (bis-NTA) groups to the re-exposed SAM areas.
The arrays of viral receptors were used for the detection of human
rhinovirus particles (serotype 2; HRV2) under native conditions by
topographical imaging at high signal-to-noise ratio. The kinetic on-rate of
the HRV2-VLDLR interaction was derived from the time-dependent
binding of the virions to the VLDL receptor spots. No significant binding
was observed for the major group virus HRV14 that uses the unrelated
receptor ICAM-1.
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