Publikation

Cell surface localised Hsp70 is a cancer specific regulator of clathrin-independent endocytosis

Outline:

B. Nimmervoll, L. Chtcheglova, K. Juhasz, N. Cremades, F. Aprile, A. Sonnleitner, P. Hinterdorfer, L. Vigh, J. Preiner, Z. Balogi - Cell surface localised Hsp70 is a cancer specific regulator of clathrin-independent endocytosis - FEBS LETTERS (Online: http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2015.07.037/full), 2015

Abstract:

The stress inducible heat shock protein 70 (Hsp70) is present specifically on the tumour cell surface yet without a pro-tumour function revealed. We show here that cell surface localised Hsp70 (sHsp70) supports clathrin-independent endocytosis (CIE) in melanoma models. Remarkably, ability of Hsp70 to cluster on lipid rafts in vitro correlated with larger nano-domain sizes of sHsp70 in high sHsp70 expressing cell membranes. Interfering with Hsp70 oligomerisation impaired sHsp70-mediated facilitation of endocytosis. Altogether our findings suggest that a sub-fraction of sHsp70 co-localising with lipid rafts enhances CIE through oligomerisation and clustering. Targeting or utilising this tumour specific mechanism may represent an additional benefit for anti-cancer therapy.