Publikation

Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells

Outline:

B. Aekbote, T. Fekete, J. Jacak, G. Vizsnyiczai, P. Ormos, L. Kelemen - Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells - Biomedical Optics Express, Vol. 7, No. 1, 2016, pp. 45-56

Abstract:

We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and non-specifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation. [less] Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells (PDF Download Available). Available from: https://www.researchgate.net/publication/286077687_Surface-modified_complex_SU-8_microstructures_for_indirect_optical_manipulation_of_single_cells [accessed Dec 17, 2015].